Thermo Fisher Scientific Phospho-ZFP36L1 (Ser334) Polyclonal Antibody
인간, 생쥐, 랫트에서 반응하는 Rabbit Polyclonal 항체로 Ser334 인산화 부위 인식. IHC, ICC/IF, ELISA 등 다양한 응용 가능. Protein A로 정제된 액상 형태로 안정적인 저장 및 높은 특이성 제공.
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Thermo Fisher Scientific Phospho-ZFP36L1 (Ser334) Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:400 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic phosphopeptide derived from human ZFP36L1 around the phosphorylation site of Ser334 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
RNA polymerase (Pol) III synthesizes tRNA, 5S rRNA, 7SL RNA, and U6 snRNA, and is overexpressed in many transformed cell lines and tumors. To maintain rapid growth, cells must produce high levels of Pol III transcribed RNA, requiring TFIIIB and TFIIIC2 transcription factor complexes.
TFIIIC2 consists of five subunits (TFIIIC220, TFIIIC110, TFIIIC102, TFIIIC90, TFIIIC63) that are overexpressed in adenovirus-transformed and malignant cells such as ovarian carcinomas. TFIIIC2 recruits RNA Pol III and TFIIIB to promoter elements and plays a key role in deregulation of malignant cells.
The TFIIIB complex includes TBP, TFIIB-related factor 1 (TFIIIB90, BRF1), and TFIIIB, which are also upregulated in transformed cells. In many carcinomas, tumor suppressors RB and p53 are inactivated, affecting their ability to bind and inactivate TFIIIB.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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