Thermo Fisher Scientific BCAR1 Monoclonal Antibody (OTI3A11), TrueMAB
BCAR1 단백질을 인식하는 Mouse IgG1 단일클론 항체로, WB, IHC, ICC, Flow 분석에 적합함. HEK293T 세포에서 발현된 인간 BCAR1 전체 길이 항원을 면역원으로 제작. 동결건조 형태로 제공되며, 재구성 시 1 mg/mL 농도 권장. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3A11 |
| Immunogen | Full-length human recombinant BCAR1 protein produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional round of desalting using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
p130Cas (Crk-associated substrate) is a cellular phosphorylation target of v-Crk and v-Src oncoproteins. It contains an SH3 domain, multiple YXXP motifs, and a proline-rich region.
p130Cas is involved in cell adhesion, migration, growth factor response, cytokine receptor signaling, and bacterial infection. Physiological functions include cardiovascular development, actin filament assembly, and Src-induced cell transformation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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