Thermo Scientific™

Klenow Fragment (10 U/μL)

Thermo Scientific Klenow Fragment is the large fragment of DNA polymerase I. It exhibits 5→3 polymerase activity and 3→5 exonuclease자세히 알아보기

Thermo Scientific Klenow Fragment is the large fragment of DNA polymerase I. It exhibits 5→3 polymerase activity and 3→5 exonuclease (proofreading) activity, but lacks 5→3 exonuclease activity of DNA polymerase I.

Highlights

• Incorporates modified nucleotides (e.g., Cy3-, Cy5-, aminoallyl-, biotin-, digoxigenin- and fluorescently-labeled nucleotides)
• Active in restriction enzyme, PCR, RT, and T4 DNA Ligase buffers

Applications
• DNA blunting by fill-in 5-overhangs • Random-primed DNA labeling • Labeling by fill-in 5-overhangs of dsDNA
• DNA sequencing by the Sanger method
• Site-specific mutagenesis of DNA with synthetic oligonucleotides
• Second strand synthesis of cDNA

사양

농도10 U/μL

중합효소DNA Polymerase I

수량300 units

제품 유형Klenow Fragment

Unit SizeEach