Thermo Scientific™

T7 DNA Polymerase (10 U/μL)

Thermo Scientific T7 DNA Polymerase, a template dependent DNA polymerase, catalyzes DNA synthesis in the 5→3 direction. It is a자세히 알아보기

Thermo Scientific T7 DNA Polymerase, a template dependent DNA polymerase, catalyzes DNA synthesis in the 5→3 direction. It is a highly processive DNA polymerase allowing continuous synthesis of long stretches of DNA. The enzyme also exhibits a high 3→5 exonuclease activity towards single- and double-stranded DNA.

Highlights

• Strong 3→5 exonuclease activity, approximately 1000-fold greater than Klenow Fragment
• Active in restriction enzyme buffers

Applications

• Purification of covalently closed circular DNA by removal of residual genomic DNA
• Primer extension reactions on long templates (see_​ Reference 1)
• DNA 3-end labeling (_see_​ Reference 1) • Strand extensions in site-directed mutagenesis • Fill-in blunting of 5-overhang DNA
• Second strand synthesis of cDNA
• _In situ detection of DNA fragmentation associated with apoptosis

Note

Assays at 37°C require only short incubation times .

사양

중합효소T7 DNA Polymerase

수량300 units

Unit SizeEach