Thermo Fisher Scientific CD62L (L-Selectin) Monoclonal Antibody (DREG.55), FITC, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-Dependent

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

DREG.55

Conjugate

FITC FITC FITC

View additional formats

• Unconjugated
• Biotin
• Request custom conjugation

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

10 µL/Test

Purification

Affinity chromatography

Storage buffer

TBS, pH 7.2-7.4, with 1% BSA

Contains

0.02% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10597456

Product Specific Information

Description: The antibody can be used to stain acetone-fixed cryostat sections or cell smears. BMS121 is not suitable for paraffin sections but is suitable as primary antibody in staining for FACS analysis.

BMS121FI can be used for direct staining of cells, BMS121BT can be used in a 2-step procedure using the biotin-(strept)avidin system.

BMS121 has not been tested in functional assays but is suitable for ELISA application.

Leukocyte-Endothelial Cell Adhesion Molecule-1 belongs to the selectin family of adhesion molecule. Together with E-selectin, P-selectin and L-selectin mediates the initial interactions of leukocytes with endothelial cells.

Selectins guide non-activated polymorphonuclear cells to the areas of inflammation in creating first, loose contacts with the endothelial layer. L-selectin in this aspect mediates rolling of PMN`s on endothelial cells. The potential binding partners of L-selectin carry a negative charge, probably a sialic acid and/or sulphate, and may contain mannose and fucose. In addition, L-selectin may also interact with E-selectin which is expressed on cytokine-activated endothelial cells. L-selectin is constitutively expressed on most leukocytes in a seemingly functional form. It is required for the binding of lymphocytes to the high endothelial venules of peripheral lymph nodes and for the invasion of neutrophils into sites of inflammation.

When neutrophils are activated, L-selectin is shed by proteolytic cleveage near the transmembrane span. Lymphocytes and monocytes can also shed L-selectin upon activation although the kinetics are significantly lower. A broad range of activating agents are effective in inducing this response. The shed form of sL-selectin is functionally active and at high concentrations can inhibit leukocyte attachment to endothelium. The main source for sL-selectin in serum seems to be tissue localized leukocytes.

Applications Tested: Flow Cytometry.

Purity: >95%.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser

Target Information

The human CD62L is a 74-95 kDa glycoprotein member of the selectin family of adhesion receptors. L-Selectin is comprised of an aminoterminal C-type lectin binding domain, an epidermal growth factor-like domain, two short consensus repeat (SCR) sequences homologous to those found in complement binding proteins, a short spacer region, a transmembrane region and a short cytoplasmic region. Human CD62L (L-Selectin) is constitutively expressed on all classes of leukocytes including lymphocytes (except a substantial population of memory T cells), monocytes and polymorphonuclear cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.