Thermo Fisher Scientific NFKB2 Polyclonal Antibody
Rabbit polyclonal antibody targeting human NFKB2/p100. Suitable for WB, IHC, ICC/IF, and IP applications. Liquid form, unconjugated, stored at -20°C. For research use only, not for diagnostic use.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:2,500–1:10,000 |
| Immunocytochemistry (ICC/IF) | 1:1,000–1:5,000 |
| Immunoprecipitation (IP) | 1–2 µL/mg of lysate |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to the N-terminus of Human NFKB2/p100 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
Target Information
The NFkB transcription factor was originally identified as a protein complex consisting of a DNA-binding subunit and an associated protein. The subunit is functionally related to c-Rel p75 and Rel B p68. The p50 subunit was initially believed to be a functionally unique protein derived from the amino-terminus of a precursor designated p105. A cDNA has been isolated that encodes an alternative DNA-binding subunit of NFkB. It is synthesized as a protein expressed in various cell types and, like p105, undergoes cleavage to generate its NFkB subunit, designated p52 (previously referred to as p49). In contrast to p50 derived from p105, p52 acts in synergy with p65 to stimulate the HIV enhancer in transiently transfected Jurkat cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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