Thermo Fisher Scientific Nestin Polyclonal Antibody
Rabbit polyclonal antibody recognizing human Nestin, a stem/progenitor cell and angiogenesis marker. Validated for WB, IHC, and IP. Suitable for research on neural stem cells, glioma, and tumor endothelial cells. Supplied as liquid, stored at -20°C.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunoprecipitation (IP) | 1–5 µL/mg of lysate |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to the center region of Human Nestin/NES |
| Conjugate | Unconjugated |
| Form | Liquid |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
Target Information
Nestin is a Class VI intermediate filament abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells. It is widely used as a marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Nestin also serves as a superior angiogenic marker to evaluate neovascularity in tumors.
Highly specific antibodies to human Nestin can help characterize progenitor cells differentiating into distinct lineages, enhance therapeutic potential of human neural stem/progenitor cells for CNS disease or injury treatment, and identify neuroepithelial tumor cells. Nestin expression has been observed in pancreatic hematopoietic stem cell populations and is considered a novel angiogenesis marker of proliferating endothelial cells in colorectal cancer tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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