Thermo Fisher Scientific BCKDE1A Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000–1:10,000

Immunoprecipitation (IP)

• Tested Dilution: 2–10 µg/mg lysate

Product Specifications

Product Specific Information

• Recommended shelf life: 1 year from date of receipt
• Based on 100% sequence identity, this antibody is predicted to react with Rat and Chimpanzee

Target Information

The BCKDHA gene encodes the E1-alpha subunit of the branched-chain alpha-keto acid (BCAA) dehydrogenase complex (BCKD; EC 1.2.4.4), an inner-mitochondrial enzyme complex that catalyzes the oxidative decarboxylation of the branched-chain alpha-ketoacids derived from isoleucine, leucine, and valine.
This reaction is the second major step in the catabolism of the branched-chain amino acids. The BCKD complex consists of three catalytic components:

1. A heterotetrameric (alpha2-beta2) branched-chain alpha-keto acid decarboxylase (E1)
2. A homo-24-meric dihydrolipoyl transacylase (E2)
3. A homodimeric dihydrolipoamide dehydrogenase (E3)

E1 is a thiamine pyrophosphate (TPP)-dependent enzyme. The reaction is irreversible and constitutes the first committed step in BCAA oxidation. The BCKDHB gene encodes the beta subunit of E1. The complex also contains two regulatory enzymes, a kinase and a phosphorylase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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