Thermo Fisher Scientific Phospho-Connexin 43 (Tyr265) Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:1,000
• Suggested Positive Control: HUVEC cells
• View 2 publications

Product Specifications

Product Specific Information

Connexin 43 antibody detects endogenous levels of Connexin 43 only when phosphorylated at Tyrosine 265.
A suggested positive control for Western blot is HUVEC cells.

Target Information

Connexin 43 (Cx43) is a member of the gap junction protein family. Connexins assemble as hexamers and are transported to the plasma membrane to create hemichannels that connect adjacent cells, forming gap junctions. These junctions are essential for cell growth regulation and development.

Phosphorylation of Cx43 regulates gap junction assembly and function. Ser368 of Cx43 is phosphorylated by PKC following activation by phorbol esters, reducing intercellular communication. Src kinase can also phosphorylate Cx43, modulating gap junction activity.

Connexin 43 is the major gap junction protein in the heart, playing a key role in synchronized cardiac contraction and embryonic development. A related pseudogene, GJA1P, is located on chromosome 5. Mutations in the GFAP gene are associated with X-linked Charcot-Marie-Tooth disease, oculodentodigital dysplasia, and heart malformations.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.