Thermo Fisher Scientific USP7 Monoclonal Antibody (5F11)

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:2,000

ELISA

• Tested Dilution: 1:10,000

Product Specifications

Product Specific Information

MA5-15585 targets HAUSP in indirect ELISA and WB applications, showing reactivity with human samples.
The immunogen is a purified recombinant fragment of human HAUSP expressed in E. coli.
MA5-15585 detects HAUSP, which has a predicted molecular weight of approximately 128 kDa.

Target Information

USP7 (also known as HAUSP) is a ubiquitin-specific protease that removes ubiquitin from its substrates. Ubiquitylation is commonly associated with protein degradation, and HAUSP activity typically stabilizes substrate proteins.
HAUSP antagonizes Mdm2, an E3 ubiquitin ligase for the tumor suppressor p53. Normally, Mdm2 mediates p53 degradation, but HAUSP can deubiquitinate and stabilize p53 in response to oncogenic stress, suggesting a tumor suppressor role.
HAUSP may also contribute to p53 activation through ARF-dependent and ARF-independent pathways, providing an alternative mechanism for cellular protection against oncogenic insults.

For Research Use Only. Not for use in diagnostic procedures or resale without express authorization.