Thermo Fisher Scientific FOXO1 Monoclonal Antibody (OTI8F12), TrueMAB

Applications

• Western Blot (WB): 1:2,000 dilution tested

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step using Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).

Target Information

FOXO1 (FKHR, Forkhead Box O1) is a transcription factor belonging to the forkhead homeotic gene family.
It can act as a coactivator or corepressor of nuclear receptor activity via the LXXLL motif in the carboxyl terminal region.
FOXO1 association with PAX3 is implicated in alveolar rhabdomyosarcoma.
It plays a role in autophagy, myogenic differentiation, and tumor suppression, and is a key target of insulin signaling regulating metabolic homeostasis under oxidative stress.
FOXO1 binds to insulin response elements (IRE) and Daf-16 family binding elements (DBE), controlling redox balance, osteoblast numbers, and bone mass.
It regulates glucose metabolism and suppresses RUNX2-mediated transcription of osteocalcin.
In hepatocytes, FOXO1 promotes gluconeogenesis with PPARGC1A by activating genes such as IGFBP1, G6PC, and PPCK1.
FOXO1 functions downstream of CDK1, PKB/AKT1, and SKT4/MST1 in regulating cell death.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.