Thermo Fisher Scientific CD11c Monoclonal Antibody (N418), PerCP-Cyanine5.5, eBioscience
마우스 CD11c를 인식하는 N418 단클론 항체로 PerCP-Cyanine5.5로 표지됨. Flow Cytometry에 최적화되어 있으며, 고순도의 액상형 제품. PBS(pH 7.2) 완충액에 보관하며 4°C 암소에서 안정적. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 2 publications |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.25 µg/test | View 140 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Armenian hamster / IgG |
| Recommended Isotype Control | Armenian Hamster IgG Isotype Control (eBio299Arm), PerCP-Cyanine5.5, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N418 |
| Conjugate | PerCP-Cyanine5.5 |
| Excitation / Emission Max | 489 / 679 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_925728 |
Additional Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV (395~805)
- Brilliant Violet (421~711)
- eFluor (450, 506, 615, 660)
- FITC
- Functional Grade
- NovaFluor Blue / Yellow Series
- PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
- Super Bright (436, 600, 645, 780)
- Request custom conjugation
Product Specific Information
The N418 monoclonal antibody reacts with mouse CD11c (integrin alpha X). CD11c non-covalently associates with beta 2 integrin to form the CD11c/CD18 heterodimer. CD11c is expressed by dendritic cells, intestinal intraepithelial lymphocytes, and some activated T cells. CD11c/CD18 binds to CD54, iC3b, and fibrinogen, playing a role in leukocyte adhesion.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of mouse splenocytes at ≤0.25 µg/test.
A test is defined as the amount of antibody to stain cells in 100 µL volume (10⁵–10⁸ cells/test).
Titrate for optimal performance.
Optical Properties:
- Excitation: 488 nm
- Emission: 695 nm
- Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD11, along with CD18, forms a heterodimer adhesion molecule.
- CD11a: Expressed in B/T lymphocytes, macrophages, monocytes, neutrophils, basophils, eosinophils
- CD11b: Expressed by monocytes, macrophages, NK cells, some B/T cells, granulocytes
- CD11c: Expressed in monocytes, macrophages, NK cells, some granulocytes, and subsets of lymphocytes
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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