Thermo Fisher Scientific SF1 Polyclonal Antibody
SF1 단백질을 인식하는 Rabbit Polyclonal 항체로, WB 및 IHC(P) 검증 완료. Human과 Mouse 시료에 반응하며, 항원 친화 크로마토그래피로 정제됨. 고순도(>95%) 제품으로 연구용에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein of human SF1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2803879 |
Product Specific Information
Purity is >95% by SDS-PAGE.
Target Information
Splicing factor 1 (SF1) is a nuclear pre-mRNA splicing factor that specifically recognizes the intron branch point sequence at the 3′ splice site, together with the large subunit of U2 auxiliary factor (U2AF). It is required for the early stages of spliceosome assembly and plays a role in nuclear pre-mRNA retention and transcriptional repression.
The protein contains:
- N-terminal U2AF ligand motif
- Central hnRNP K homology motif and quaking 2 region (binds branch-site adenosine)
- Zinc knuckles domain
- C-terminal proline-rich domain
Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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