Thermo Fisher Scientific NEFL Polyclonal Antibody
인간 NEFL 단백질을 인식하는 Rabbit Polyclonal 항체로, IHC, ICC/IF, ELISA, IP에 사용 가능. Protein G로 정제된 액상 형태이며 4 mg/mL 농도. 신경세포의 중간 필라멘트 단백질 연구에 적합. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:20–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | Assay-dependent |
| Immunoprecipitation (IP) | 1:200–1:2,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant Human Neurofilament light polypeptide protein (128–271AA) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 4 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.03% Proclin 300 |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Wet ice |
| RRID | AB_3091481 |
Target Information
Neurofilaments are intermediate filament proteins found specifically in neurons and are essential for maintaining neuronal caliber. They consist mainly of three major proteins: NF-L (light), NF-M (medium), and NF-H (heavy).
NF-L is the low molecular weight subunit (~70 kDa) and plays a key role in neuronal structure. NF-H, the heavy subunit, runs at approximately 180–220 kDa on SDS-PAGE, with some interspecies variation.
Expression of these intermediate filament proteins is tissue-specific and developmentally regulated.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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