Thermo Fisher Scientific E2F1 Polyclonal Antibody
E2F1 전사인자에 특이적인 Rabbit Polyclonal Antibody로, WB, IHC, ELISA, IP 등 다양한 응용에 적합합니다. Human 및 Mouse 시료에 반응하며, 고순도의 Protein G 정제 항체로 안정적인 결과를 제공합니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:5,000 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:20–1:200 | - |
| ELISA | Assay-dependent | - |
| Immunoprecipitation (IP) | 1:200–1:2,000 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant Human Transcription factor E2F1 protein (133–362 AA) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 3.5 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.03% Proclin 300 |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Wet ice |
| RRID | AB_3093517 |
Target Information
The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of the cell cycle and the action of tumor suppressor proteins, and is also a target of transforming proteins of small DNA tumor viruses.
E2F proteins contain several conserved domains, including:
- DNA binding domain
- Dimerization domain (interacts with DP transcription factors)
- Transactivation domain enriched in acidic amino acids
- Tumor suppressor protein association domain (within transactivation domain)
E2F1, along with E2F2 and E2F3, includes an additional cyclin binding domain. It binds preferentially to retinoblastoma protein (pRB) in a cell cycle-dependent manner and can mediate both cell proliferation and p53-dependent/independent apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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