Thermo Fisher Scientific RB1 Monoclonal Antibody (OTI11C5), TrueMAB
RB1 단백질을 표적으로 하는 마우스 단클론 항체로, Western blot에 최적화됨. Human 반응성, carrier-free 형태로 재결합 및 결합 실험에 적합. 종양 억제 단백질 Rb 연구용으로 사용 가능. 연구용으로만 사용.
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Applications
- Western Blot (WB): Tested dilution 1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI11C5 |
| Immunogen | Human recombinant protein fragment (amino acids 309–590 of human RB1) produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Rb is a tumor suppressor nuclear phosphoprotein capable of binding to DNA. It is phosphorylated on serine and threonine residues but not on tyrosine. Rb forms complexes with SV40 large T antigen, adenovirus E1A, and human papilloma virus-16 E.
Loss of Rb function leads to uncontrolled cell growth, and RB gene aberrations are implicated in cancers of the breast, colon, prostate, kidney, nasopharynx, and leukemia.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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