Thermo Fisher Scientific TIP47 Polyclonal Antibody
Human PLIN3 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, ICC/IF 등 다양한 응용에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, PBS/glycerol buffer에 보관. TIP47 단백질의 세포 내 수송 및 암 관련 연구에 적합.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:1,000 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human PLIN3. Recombinant protein control fragment (Product #RP-88959). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.35 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2789408 |
Product Specific Information
Immunogen sequence:
SLGKLRATKQ RAQEALLQLS QALSLMETVK QGVDQKLVEG QEKLHQMWLS WNQKQLQGPE KEPPKPEQVE SRALTMFRDI AQQLQATCTS LGSSIQGLPT NVKDQVQQAR RQVEDLQATF SSIHS
Target Information
TIP47 has been described as a cargo protein involved in the trafficking of the mannose-6-phosphate receptor between endosomes and the Golgi complex. Mannose 6-phosphate receptors (MPRs) deliver lysosomal hydrolase from the Golgi to endosomes and then return to the Golgi complex.
The protein encoded by this gene interacts with the cytoplasmic domains of both cation-independent and cation-dependent MPRs and is required for endosome to Golgi transport. This protein also binds directly to the GTPase RAB9, a member of the RAS oncogene family.
The interaction with RAB9 has been shown to increase the affinity of this protein for its cargo. TIP47 has been localized in milk fat globule membranes of human and bovine origin and described as a placental protein. Increased amounts of TIP47 are secreted into circulation of cervix carcinoma patients, suggesting its significance as an oncodevelopmental marker.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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