Thermo Fisher Scientific Methyl-p53 (Lys372) Polyclonal Antibody
p53 단백질의 Lys372 메틸화 형태를 인식하는 Rabbit Polyclonal Antibody. Western blot, IHC, ICC/IF 등 다양한 응용 가능. 높은 특이성과 재현성을 제공하며, Human, Rodent, Amphibian 등에서 반응. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Amphibian, Human, Rodent |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to a region within amino acids 312–404 of Human p53 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.43 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 1% BSA, 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2548421 |
Product Specific Information
- Recommended positive controls: HCT116 24hr cisplatin 30 µM, 48hr cisplatin µM
- Predicted reactivity: Sheep (100%), Rhesus Monkey (100%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The tumor suppressor protein p53 is a sequence-specific transcription factor activated by cellular stress. It mediates cell cycle arrest or apoptosis in response to DNA damage or metabolic stress.
p53 regulates transcription of genes such as p21waf1, contributing to G1 or G2/M arrest. Structurally, p53 includes an N-terminal transactivation domain, a central DNA-binding domain, an oligomerization domain, and a C-terminal regulatory domain.
Phosphorylation at Ser15 is critical for activation and stabilization, while Ser392 phosphorylation is associated with tumor formation. p53 plays a key role in preventing proliferation of damaged cells, acting as an anticancer factor.
Alterations of the TP53 gene are common in human malignancies and can occur as somatic or germline mutations, such as in Li-Fraumeni syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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