Thermo Fisher Scientific Methyl-p53 (Lys372) Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Recommended positive controls: HCT116 24hr cisplatin 30 µM, 48hr cisplatin µM
• Predicted reactivity: Sheep (100%), Rhesus Monkey (100%)
• Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The tumor suppressor protein p53 is a sequence-specific transcription factor activated by cellular stress. It mediates cell cycle arrest or apoptosis in response to DNA damage or metabolic stress.
p53 regulates transcription of genes such as p21waf1, contributing to G1 or G2/M arrest. Structurally, p53 includes an N-terminal transactivation domain, a central DNA-binding domain, an oligomerization domain, and a C-terminal regulatory domain.

Phosphorylation at Ser15 is critical for activation and stabilization, while Ser392 phosphorylation is associated with tumor formation. p53 plays a key role in preventing proliferation of damaged cells, acting as an anticancer factor.
Alterations of the TP53 gene are common in human malignancies and can occur as somatic or germline mutations, such as in Li-Fraumeni syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.