Thermo Fisher Scientific HRAS Monoclonal Antibody (OTI1D9), TrueMAB
HRAS 단백질을 표적하는 Mouse IgG1 단일클론 항체로, WB, IHC, ICC/IF에 사용 가능. 인간, 마우스, 랫, 개에 반응하며, 고순도 친화 크로마토그래피 정제. 연구용으로 Ras 관련 신호전달 및 암 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1D9 |
| Immunogen | Full length human recombinant HRAS protein produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Monomeric G protein Ras acts as a molecular switch linking receptor and nonreceptor tyrosine kinase activation to downstream signaling pathways that lead to distinct cytoplasmic or nuclear events. Mammalian cells contain three Ras proto-oncogenes coding for closely related Ras proteins: H-, K-, and N-Ras. Oncogenic mutations in Ras genes occur in approximately 30% of human cancers. Constitutive activation of Ras due to mutations or overexpression promotes proliferation and inhibits apoptosis.
- K-Ras mutations: pancreatic, colorectal, and non-small-cell lung carcinomas
- H-Ras mutations: bladder, kidney, and thyroid carcinomas
- N-Ras mutations: melanoma, hematological malignancies, and hepatocellular carcinoma
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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