Thermo Fisher Scientific DHFR Monoclonal Antibody (2B10)
DHFR 단백질을 인식하는 마우스 단클론 항체. Western blot, ICC/IF, ELISA에 사용 가능. 인간 및 랫트 반응성. 친화 크로마토그래피로 정제된 액상 항체로, PBS(pH 7.4) 버퍼에 보존제 없이 제공. 연구용 전용 제품.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–5 µg/mL |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
| ELISA | 0.1 ng/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2B10 |
| Immunogen | DHFR (AAH03584, 88 a.a. ~ 187 a.a.) partial recombinant protein with GST tag (MW of GST tag: 26 kDa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Sequence of this protein is as follows:
HFLSRSLDDA LKLTEQPELA NKVDMVWIVG GSSVYKEAMN HPGHLKLFVT RIMQDFESDT FFPEIDLEKY KLLPEYPGVL SDVQEEKGIK YKFEVYEKND
Target Information
Dihydrofolate reductase (DHFR) converts dihydrofolate into tetrahydrofolate and is required for the synthesis of purines, thymidylic acid, and certain amino acids. The functional DHFR gene is located on chromosome 5, while multiple intronless processed pseudogenes or DHFR-like genes exist on other chromosomes. DHFR deficiency has been associated with megaloblastic anemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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