Thermo Fisher Scientific Perforin-1 (Pore Forming Protein) Monoclonal Antibody (PRF1, 2467)

Applications

ELISA (ELISA)

• Tested Dilution: Assay-dependent

Peptide Array (Array)

• Tested Dilution: Assay-dependent

Product Specifications

Product Specific Information

• Antibody is tested in direct ELISA.
• This antibody has only been tested in direct ELISA.

Target Information

Perforin is one of the major cytolytic proteins of cytolytic granules. It acts as a cytolytic mediator stored in and released by cytoplasmic granules. Perforin plays a crucial role in immune defense against tumors and viral infections mediated by cytotoxic lymphocytes.

Perforin is a 555 amino acid protein with a 21 amino acid signal peptide and has a molecular weight of approximately 70–75 kDa. It is a pore-forming protein with a mechanism of transmembrane channel formation similar to complement component C9, and homology between perforin and C9 has been demonstrated.

Studies show that perforin is expressed only in killer cell lines, not in helper T lymphocytes or other tumor cells. It is a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Mutations in the perforin gene are associated with familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare, lethal autosomal recessive disorder of early childhood. Alternative splicing results in multiple transcript variants of perforin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.