Thermo Fisher Scientific CD223 (LAG-3) Monoclonal Antibody (3DS223H), Brilliant Violet 605, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.5 µg)/test

Product Specifications

Available Conjugate Formats

• Alexa Fluor 647, 660, 700
• APC, APC-eFluor 780
• Brilliant UV 395, 563, 661, 737, 805
• Brilliant Violet 421, 480, 650, 711, 786
• eFluor 450, 506
• FITC
• NovaFluor Blue, Red, Yellow series
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation

Product Specific Information

This 3DS223H monoclonal antibody recognizes human CD223, also known as Lymphocyte Activation Gene 3 (LAG-3).
LAG-3 is a 70-kDa surface glycoprotein of the Ig superfamily, homologous to CD4, and binds MHC class II with higher affinity than CD4. It plays a role in negative regulation of T cell activation and homeostatic proliferation.
LAG-3 is expressed on activated T cells (including regulatory T cells) and NK cells, with CD8+ T cells showing higher expression than CD4+ T cells. Coexpression of LAG-3 and CD49b may identify Type 1 regulatory T cells (Tr1).

Experimental Notes

• Recognizes a formaldehyde-fixed epitope.
• Reported for use in flow cytometric analysis.
• Pre-diluted and tested on normal human peripheral blood cells.
• Recommended usage: 5 µL (0.5 µg) per test (100 µL total volume).
• Compatible with violet (405 nm) laser-equipped cytometers.

Handling and Staining

• Use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize nonspecific polymer interactions.
• Protect from light to prevent photo-induced oxidation.
• Fixation: Samples may be stored in IC Fixation Buffer (00-8222-49) or 1-step Fix/Lyse Solution (00-5333-54) for up to 3 days at 4°C in the dark.
• Not compatible with methanol-based fixation.

Excitation: 407 nm
Emission: 605 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

LAG-3 is a 70-kDa surface glycoprotein of the Ig superfamily, homologous to CD4, binding MHC class II with high affinity. It is involved in negative regulation of T cell activation and proliferation, expressed on activated T cells and NK cells. Coexpression with CD49b identifies Type 1 regulatory T cells (Tr1).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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