Thermo Fisher Scientific PRDX5 Monoclonal Antibody (OTI3B6), TrueMAB
PRDX5 단백질을 인식하는 단클론 항체로, Western blot 및 면역조직화학(IHC)에 적합. Mouse, Rat, Human 반응성. 동결건조 형태로 제공되며, PBS 기반 버퍼에 보존제 무첨가. 연구용으로 세포 증식 및 산화환원 관련 연구에 활용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:250 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse, Rat, Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3B6 |
| Immunogen | Full length human recombinant protein of human PRDX5 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Peroxiredoxin (Prx) is a family of peroxidases involved in cell proliferation, differentiation, and apoptosis.
Mammalian Prx proteins are categorized into typical 2-Cys, atypical 2-Cys, and 1-Cys types.
Except for Prx VI (1-Cys subgroup), the other five 2-Cys Prx isoforms exhibit thioredoxin-dependent peroxidase activity utilizing thioredoxin, thioredoxin reductase, and NADPH as a reducing system.
Mammalian Prxs (20–30 kDa) differ in subcellular localization:
- Prx I, II, VI: Cytosol
- Prx III: Mitochondria
- Prx IV: Endoplasmic reticulum and secretion
- Prx V: Peroxisome, mitochondria, and cytosol
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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