Thermo Fisher Scientific EphB4 Recombinant Rabbit Monoclonal Antibody (001)
Human EphB4 단백질을 인식하는 Thermo Fisher의 재조합 토끼 단일클론 항체입니다. ICC/IF, Flow Cytometry, ELISA에 사용 가능하며, 높은 특이성과 재현성을 제공합니다. 보존제가 없으며, 장기 보관 시 -20°C에서 보관을 권장합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:25–1:100 |
| ELISA | 1:5,000–1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 001 |
| Immunogen | Recombinant Human EphB4/Eph Receptor B4 protein (Met1–Ala539) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C (avoid freeze/thaw cycles) |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2785147 |
Available Conjugated Formats
Product Specific Information
This product is preservative-free. It is recommended to add sodium azide (final concentration 0.05%–0.1%) to prevent contamination.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. Antibody DNA sequences from immunoreactive rabbits are cloned, and individual clones are screened to select optimal candidates for production.
Advantages of recombinant rabbit monoclonal antibodies:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to larger rabbit immune repertoire
This antibody has specificity for Human EphB4/Eph Receptor B4.
Target Information
EPHB4 is a receptor tyrosine kinase belonging to the ephrin receptor family. Eph kinases play crucial roles in nervous system development, angiogenesis, and synapse formation. Ephrin ligands are divided into ephrin-A (EFNA) and ephrin-B (EFNB) classes, with Eph receptors grouped accordingly based on extracellular domain similarities and ligand affinities.
Ephrin receptors represent the largest subgroup of receptor tyrosine kinases (RTKs). EphB4 binds to ephrin-B2 and is essential for vascular development.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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