LanthaScreen™ TR-FRET PPAR alpha Competitive Binding Assay Kit, goat

This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15143:The LanthaScreen™ TR-FRET PPAR alpha Competitive자세히 알아보기

This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15143:

The LanthaScreen™ TR-FRET PPAR alpha Competitive Binding Assay provides a sensitive and robust method for high-throughput screening (HTS) of ligands for peroxisome proliferator-activated receptor alpha (PPAR alpha). The kit uses a terbium-labeled anti-GST antibody, a fluorescent small-molecule pan-PPAR ligand (Fluormone™ Pan-PPAR Green), and a human PPAR alpha ligand-binding domain (LBD) that is tagged with glutathione S-transferase (GST), in a homogeneous mix-and-read assay format.

*To assay:
*When running the LanthaScreen™ TR-FRET PPAR alpha Competitive Binding Assay, Fluormone™ Pan-PPAR Green is added to ligand test compounds followed by addition of a mixture of the PPAR alpha-LBD and terbium anti-GST antibody. When the Fluormone™ Pan-PPAR Green is bound to PPAR alpha, energy transfer from the terbium-labeled antibody to the tracer occurs, and a high TR-FRET ratio is observed. Competitive ligand binding to PPAR alpha is detected by a test compound`s ability to displace the tracer, which results in a loss of FRET between the antibody and the tracer. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and can be used to determine the IC50 from a dose response curve of the compound (Figure 1). This type of binding assay is analogous to radioligandbased assays, except that it eliminates handling of radioactivity and enables a homogeneous “addition-only” format.

사양

콘주게이트Tb (Terbium)

검출 방법Fluorescence

용도(장비)Microplate Reader

수량400 x 40 μL

포장384-well Plate

어세이 입력Biochemical competitive binding

판독End Point

배송 조건Dry Ice

타겟 입력PPARA, PPAR alpha, NR1C1

제품라인LanthaScreen™

용도(애플리케이션)Competitive Binding Assay, TR-FRET

리간드Pan-PPAR

제품 유형TR-FRET PPAR Alpha Competitive Binding Assay Kit

Unit SizeEach