Thermo Fisher Scientific Kinesin-1 Polyclonal Antibody
Kinesin-1 단백질을 인식하는 토끼 다클론 항체로, Western blot, IHC, ELISA, IP 등에 사용 가능. 인간 시료에 반응하며, 액상 형태로 제공. -20°C에서 보관하며, 반복적인 동결-해동은 피해야 함.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:100–1:500 |
| Immunohistochemistry (IHC) | Assay-dependent |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Anti-Kinesin-1 was prepared from whole rabbit serum produced by repeated immunizations with a truncated kinesin-1 construct expressed in E. coli corresponding to human kinesin-1 protein. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 77 mg/mL |
| Storage Buffer | 0.02M potassium phosphate/whole serum, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Anti-Kinesin-1 is directed against the human kinesin-1 protein. A BLAST analysis was used to suggest reactivity with human. Cross-reactivity with kinesin-1 from other sources has not been determined.
Target Information
Kinesin-1 is a microtubule-dependent motor protein required for normal distribution of mitochondria and lysosomes. It can induce formation of neurite-like membrane protrusions in non-neuronal cells in a ZFYVE27-dependent manner. It regulates centrosome and nuclear positioning during mitotic entry. During the G2 phase of the cell cycle, in a BICD2-dependent manner, it antagonizes dynein function and drives the separation of nuclei and centrosomes. It is required for anterograde axonal transport of MAPK8IP3/JIP3, which is essential for axon elongation.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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