Thermo Fisher Scientific HPV Type 16 E7 Monoclonal Antibody (716-281)

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

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ELISA (ELISA)

Assay-dependent

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Product Specifications

Published species

Virus

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

716-281

Immunogen

Full size recombinant E7 protein of HPV type 16

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2.6 mg/mL

Storage conditions

4° C, do not freeze

Shipping conditions

Wet ice

RRID

AB_559524

Product Specific Information

Predicted molecular weight of 11 kDA.

Target Information

The HPV E7 proteins are small (HPV16 E7 comprising 98 amino acids, 15kDa), zinc binding phosphoproteins which are localised in the nucleus. They are structurally and functionally similar to the E1A protein of subgenus C adenoviruses. The first 16 amino-terminal amino acids of HPV16 E7 contain a region homologous to a segment of the conserved region 1 (CR1) of the E1A protein of subgenus C adenoviruses. The next domain, up to amino acid 37, is homologous to the entire region 2 (CR2) of E1A. Genetic studies have established that these domains are required for cell transformation in vitro, suggesting similarities in the mechanism of transformation by these viruses. The CR2 homology region contains the LXCXE motif (residues 22-26) involved in binding to the tumour suppressor protein pRb.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.