Thermo Fisher Scientific TSG101 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 19 publications 19 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

-

Immunocytochemistry (ICC/IF)

1:100

-

ELISA (ELISA)

1:1,000-1:10,000

-

Immunoprecipitation (IP)

1:100-1:500

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Bovine, Horse, Human, Mouse, Zebrafish

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein fragment corresponding to a region within amino acids 117 and 324 of Human TSG101 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:TSG101,uniProtId:Q99816-1,ncbiNodeId:9606,antigenRange:117-324,antigenLength:390,antigenImageFileName:PA5-31260_TSG101_Q99816-1_Rabbit.svg,antigenImageFileNamePDP:PA5-31260_TSG101_Q99816-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.42 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2548734

Product Specific Information

Recommended positive controls: 293T, A431, HeLa, HepG2, K562, THP-1, HL-60, Neuro 2A, C8D30, NIH-3T3, Raw264.7, C2C12, A549, H1299, HCT116, U87-MG, SK-N-SH, IMR32, SK-N-AS.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

TSG101-4A10 recognizes TSG101 protein, the product of a recently identified Tumor Susceptibility Gene whose inactivation in mouse fibroblasts results in cell transformation and the ability of those cells to form tumors in nude mice. The TSG101 gene encodes a 46 kDa protein and contains a proline-rich domain and a leucine-zipper motif within a coiled-coil domain near the carboxyl terminus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.