
Thermo Fisher Scientific CSTF77 Polyclonal Antibody
Rabbit polyclonal antibody targeting human CSTF3 (CSTF77) for WB, IHC, and IP applications. Recognizes residues 1–50 of CSTF3. Supplied as unconjugated liquid, 0.20 mg/mL concentration. Stored at 4°C, shipped on wet ice. Research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 1 and 50 of human cleavage stimulation factor, 3′ pre-RNA, subunit 3, 77 kDa |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt.
- Application Note: For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections.
- Based on 100% sequence identity, this antibody is predicted to react with Orangutan.
Target Information
The CSTF3 gene encodes one of three cleavage stimulation factors (CSTF1, CSTF2, and CSTF3) that form the CSTF complex. This complex is involved in polyadenylation and 3′ end cleavage of pre-mRNAs. The CSTF3 protein functions as a homodimer and interacts with both CSTF1 and CSTF2 within the complex. Alternative splicing produces multiple transcript variants encoding different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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