Thermo Fisher Scientific TCF12 Polyclonal Antibody
TCF12 단백질을 인식하는 Rabbit Polyclonal 항체로 Western blot 및 Immunocytochemistry에 사용 가능. 인간 시료에 반응하며, 항원 친화 크로마토그래피로 정제됨. PBS 기반 용액 형태로 제공되며 4°C 또는 -20°C에서 보관.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant Human TCF12. Recombinant protein control fragment (Product # RP-107458) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.05 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2664505 |
Product Specific Information
Immunogen sequence:
FSPPVNSGKTR PTTLGSSQFS GSGIDERGGT TSWGTSGQPS PSYDSSRGFT DSPHYSDHLN DSRLGAHEGL SPTPFMNSNL MGKTSERGSF SLYSRDTGLP GCQSSLLRQD LGLGSPAQLS SSGKPGTAYY SFSATSSRRR P
Highest antigen sequence identity to the following orthologs:
- Mouse: 96%
- Rat: 96%
Target Information
TCF12 (Transcription Factor 12), also known as HTF4, is a member of the basic helix-loop-helix (bHLH) E-protein family that recognizes the consensus binding site (E-box) CANNTG.
It is expressed in multiple tissues including skeletal muscle, thymus, and B/T cells, and plays a role in regulating lineage-specific gene expression through heterodimer formation with other bHLH E-proteins.
TCF12, in combination with E2A, is required to block thymocyte proliferation prior to pre-TCR expression and is critical for proper T cell differentiation.
Recent studies indicate that TCF12 is essential for the development of invariant natural killer T cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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