Thermo Fisher Scientific NFkB p65 Polyclonal Antibody
NFkB p65 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot과 IHC(Paraffin) 실험에 적합합니다. Human, Rabbit, Rat 시료에 반응하며, 항원 친화 크로마토그래피로 정제되었습니다. NF-kB 신호전달 연구에 유용합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–10 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Rabbit, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide derived from internal domain of human NFkB p65 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: Breast carcinoma
- Cellular location: Nuclear
Target Information
Nuclear factor kB p65 (NF-kB p65) is encoded by the RELA gene and is located on chromosome 11 in humans. NF-kB p65, also known as RelA, belongs to the Rel family of proteins. It forms a heterodimer with other NF-kB subunits such as p50 or p52. Upon TNF alpha stimulation, IKK phosphorylates IκB, leading to its proteasomal degradation and allowing NF-kB translocation to the nucleus. NF-kB p65 plays a crucial role in the transcription of the immunoglobulin kappa gene in mature B-lymphoid cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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