Invitrogen™

CellROX™ Deep Red Flow Cytometry Assay Kit

The CellROX™ Deep Red Flow Cytometry Assay Kit enables the flow cytometric detection of reactive oxygen species (ROS) in live자세히 알아보기

The CellROX™ Deep Red Flow Cytometry Assay Kit enables the flow cytometric detection of reactive oxygen species (ROS) in live cells. The kit includes the novel, fluorogenic CellROX™ Deep Red Reagent, as well as SYTOX™ Blue dead cell stain, N-acetyl cysteine (an antioxidant, for negative control), and tert-butyl hydroperoxide solution (TBHP, an inducer of ROS).

View a selection guide for all CellROX™ Reagents and Kits.

The CellROX™ Deep Red Flow Cytometry Assay Kit features:

• Fluorogenic probe formulated for flow cytometry that is oxidized in the presence of ROS
• Multicolor compatibility—minimal overlap with fluorophores excited by other laser lines, allowing easy multiplexing with other reagents
• Simple protocol—cells can be stained in complete media or other appropriate buffer, no need for serum free media

The CellROX™ Deep Red Detection Reagent is cell-permeable and non-fluorescent or very weakly fluorescent while in the reduced state. Upon oxidation, the reagent exhibits a strong fluorogenic signal that has absorption/emission maxima of 644/665 nm and remains localized in the cytoplasm. When used together with the included SYTOX™ Blue Dead Cell Stain, oxidatively stressed and non-stressed cells are reliably distinguished from dead cells by flow cytometry.

사양

세포 유형Mammalian Cells, Eukaryotic Cells

검출 방법Fluorescence

용도(애플리케이션)Flow Cytometry

용도 (장비)Flow Cytometer

제품 유형Reagent

염료 유형CellROX™ Deep Red Reagent

방출CellROX™ Deep Red: 644⁄665, SYTOX™ blue: 444⁄480

형식Tube(s)

제품라인CellROX™

수량100 Assays

배송 조건Wet Ice

용해도DMSO (Dimethylsulfoxide)

Unit SizeEach