Thermo Fisher Scientific eIF3c Polyclonal Antibody
Rabbit polyclonal antibody against human eIF3c for IHC and ICC/IF applications. Validated for human samples with high specificity. Supplied as liquid form, antigen affinity purified, and stored in PBS with glycerol. For research use only.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:20–1:50 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human eIF3c. Recombinant protein control fragment (Product # RP-104421) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.10 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2640959 |
Product Specific Information
Immunogen sequence:
YYKFDYKAHQ RQLTPPEGSS KSEQDQAENE GEDSAVLMER LCKYIYAKDR TDRIRTCAIL CHIYHHALHS
Antigen sequence identity:
Mouse – 100%
Rat – 100%
Target Information
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. It is also required for disassembly and recycling of post-termination ribosomal complexes, preventing premature joining of the 40S and 60S ribosomal subunits prior to initiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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