Thermo Fisher Scientific NELFE Polyclonal Antibody
NELFE 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, IP 등에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, 1 mg/mL 농도의 액상 형태. 인간 단백질에 반응하며, RNA polymerase II 전사 신장 억제 복합체 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 330 and 380 of human negative elongation factor polypeptide E |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Tris citrate/phosphate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections
- Based on 100% sequence identity, this antibody is predicted to react with Bovine
Target Information
RDBP is part of a complex termed negative elongation factor (NELF), which represses RNA polymerase II transcript elongation. This protein shows similarity to nuclear RNA-binding proteins, though RNA-binding has not been demonstrated. It contains alternating basic and acidic residues (arginine and aspartic acid). The gene is localized to the MHC class III region on chromosome 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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