Thermo Fisher Scientific Ki-67 Monoclonal Antibody (SolA15), PE-Cyanine5, eBioscience
Ki-67 단백질을 인식하는 SolA15 클론 단일클론 항체로 세포 증식 마커 분석에 적합. 다양한 종(인간, 마우스, 랫, 개 등)에 반응하며 Flow Cytometry 및 IHC에 사용 가능. PE-Cyanine5 형광 표지로 높은 감도 제공. 실험용, 4°C 암소 보관.
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Thermo Fisher Scientific Ki-67 Monoclonal Antibody (SolA15), PE-Cyanine5, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | 14 |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | 1 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | - | 1 |
| Immunohistochemistry (Frozen) (IHC (F)) | - | 1 |
| Immunocytochemistry (ICC/IF) | - | 10 |
| Flow Cytometry (Flow) | 0.5 µg/test | 14 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Cynomolgus monkey, Human, Mouse, Non-human primate, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine5, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SolA15 |
| Conjugate | PE-Cyanine5 |
| Excitation/Emission Max | 568 / 666 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2802209 |
Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 660, 700, Plus 594
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 395–805 series
- Brilliant Violet 421–786 series
- eFluor 450, 506, 570, 615, 660
- FITC, PE, PE-Cyanine7, PE-eFluor 610, PerCP-eFluor 710
- Custom conjugation available
Product Specific Information
The monoclonal antibody SolA15 recognizes mouse and rat Ki-67, a 300 kDa nuclear protein present during all active phases of the cell cycle (G1, S, G2, and mitosis), but absent in resting cells (G0). Ki-67 redistributes from the nucleus to chromosomes during mitosis and is widely used as a proliferation marker to assess cell growth fraction.
SolA15 also recognizes human, non-human primate, and canine Ki-67.
Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Validated using stimulated mouse splenocytes with the Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00). Recommended ≤0.5 µg/test (100 µL final volume). Optimal antibody titration advised.
Light Sensitivity: Protect vial and stained samples from light.
Fixation: Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-57). Clone-specific performance should be verified empirically.
Excitation: 488–561 nm
Emission: 667 nm
Compatible Lasers: Blue, Green, Yellow-Green
Target Information
Ki-67 is a nuclear protein expressed during late G1, S, G2, and M phases of the cell cycle. It associates with euchromatin and heterochromatin structures and is absent in G0 cells. Ki-67 antibodies are used to determine the proliferative fraction of cells in tumors, correlating with tumor grade and prognosis. Commonly used as a neuronal and neoplastic proliferation marker.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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