Thermo Fisher Scientific JMJD3 Polyclonal Antibody
JMJD3 단백질을 인식하는 Rabbit Polyclonal 항체로, WB 및 IHC(P) 실험에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, Human에 반응하고 Mouse 및 Rat에서도 예측 반응성을 보입니다. 연구용으로 4°C 또는 -20°C에서 보관 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 1100–1358 of Human JMJD3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.95 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2549665 |
Product Specific Information
- Recommended positive controls: 293T, A375
- Predicted reactivity: Mouse (98%), Rat (99%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Covalent modification of histones plays a critical role in regulating chromatin structure and transcription. While most covalent histone modifications are reversible, it has been established that methyl groups are also subject to enzymatic removal from histones.
A family of novel JmjC domain-containing histone demethylation (JHDM) enzymes perform this function. Histone demethylation by JHDM proteins requires cofactors Fe(II) and alpha-ketoglutarate.
Family members include JHDM1 (demethylating histone 3 at lysine 36), and JHDM2A as well as JMJD2C H3K9 (both demethylate histone 3 at lysine 9).
Histone demethylase activity has been linked to tumor development, reduced cell proliferation, and hormone-dependent transcriptional activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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