Thermo Fisher Scientific CD19 Monoclonal Antibody (HIB19), NovaFluor Blue 530, eBioscience

Thermo Fisher Scientific CD19 Monoclonal Antibody (HIB19), NovaFluor Blue 530, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 4 µL (0.6 µg)/test

Product Specifications

Additional Formats Available

Unconjugated, Alexa Fluor 488, Alexa Fluor 532, Alexa Fluor 561, Alexa Fluor 660, Alexa Fluor 700, APC, APC-eFluor 780, Biotin, Brilliant UV/Violet/Yellow series, PE, PE-Cyanine5, PE-Cyanine7, PerCP conjugates, Super Bright series, and NovaFluor variants.
Custom conjugation available upon request.

Product Specific Information

The HIB19 monoclonal antibody reacts with human CD19, a 95 kDa transmembrane glycoprotein expressed on B cells during all stages of development except plasma cells. Follicular dendritic cells also express CD19. CD19 forms a complex with CD21, CD81, Leu13, and MHC class II, associated with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux, and B cell proliferation.
This antibody is validated for flow cytometric analysis of normal human peripheral blood cells and can be used at 4 µL (0.6 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Compatibility Note:
NovaFluor dyes are not compatible with DNA intercalating viability dyes such as propidium iodide, 7-AAD, and DAPI. Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability analysis.

Recommended Buffer:
Each NovaFluor conjugate ships with CellBlox Blocking Buffer.

• Add 5 µL per sample when staining with NovaFluor conjugates.
• For single-color controls, use 5 µL per 100 µL cell sample (10³–10⁸ cells).

Spectral Properties:
Excitation: 509 nm; Emission: 530 nm; Laser: 488 nm (Blue)

NovaFluor conjugates utilize Phiton™ technology based on nucleic acid dye structures engineered for optimized fluorescence with reduced spillover and spread.

Target Information

CD19 is a member of the immunoglobulin superfamily with two Ig-like domains. It is expressed on all peripheral B cells and certain myeloid leukemia cells. CD19 acts as a signal-amplifying coreceptor for the B cell receptor (BCR) and regulates normal and malignant B cell proliferation.
Mutation of CD19 leads to hypogammaglobulinemia, while overexpression causes B cell hyperactivity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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