Thermo Fisher Scientific POLR2H Monoclonal Antibody (OTI1C10), TrueMAB
POLR2H 단백질을 인식하는 마우스 단일클론 항체로, Western blot에 적합. Human, Mouse, Rat에 반응하며, 항원은 E.coli에서 생산된 POLR2H(aa 2-150) 재조합 단백질. 무보존제, 동결건조 형태로 제공되며, 연구용으로 사용.
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Thermo Fisher Scientific POLR2H Monoclonal Antibody (OTI1C10), TrueMAB
Applications and Tested Dilution
- Western Blot (WB): 1:500–1:2,000
Species Reactivity
- Human
- Mouse
- Rat
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1C10 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 2–150 of human POLR2H produced in E.coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Eukaryotic cells contain three distinct RNA polymerases—Pol I, II, and III—each responsible for synthesizing different classes of RNA:
- Pol I: Transcribes rRNA genes for the 28S, 18S, and 5.8S ribosomal RNA precursors.
- Pol II: Transcribes protein-coding genes into mRNA and snRNA genes into snRNAs involved in RNA processing.
- Pol III: Transcribes 5S rRNA and all tRNA genes.
Each polymerase consists of 9–15 polypeptides, with RPB6 and RPB8 subunits shared among all three.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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