Thermo Fisher Scientific SF1 Polyclonal Antibody
Thermo Fisher Scientific SF1 Polyclonal Antibody는 인간 SF1 단백질을 인식하는 토끼 IgG 기반 항체로, IHC(P) 및 ICC/IF 실험에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, PBS buffer와 glycerol로 안정화되어 장기 보관이 가능합니다.
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Applications
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:200–1:500
Immunocytochemistry (ICC/IF)
- Tested Dilution: 0.25–2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human SF1. Recombinant protein control fragment (Product #RP-100742). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.05 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2647172 |
Product Specific Information
Immunogen sequence:
NQDTMEQKTV IPGMPTVIPP GLTREQERAY IVQLQIEDLT RKLRTGDLGI PPNPEDRSPS PEPIYNSEGK RLNTREFRTR KKLEEERHNL ITEMVALNPD FKPPADYKPP ATRVSDKVMI PQDEYPEINF VGLLI
- Highest antigen sequence identity to orthologs: Mouse – 99%, Rat – 99%.
Target Information
Splicing factor 1 (SF1) is a nuclear pre-mRNA splicing factor that specifically recognizes the intron branch point sequence at the 3′ splice site together with the large subunit of U2 auxiliary factor (U2AF). It is essential for the early stages of spliceosome assembly and also plays roles in nuclear pre-mRNA retention and transcriptional repression.
The protein contains:
- N-terminal U2AF ligand motif
- Central hnRNP K homology motif and quaking 2 region (binds branch-site adenosine)
- Zinc knuckles domain
- C-terminal proline-rich domain
Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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