Thermo Fisher Scientific EDPF1 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: Assay-dependent

Product Specifications

Target Information

The differentiation and activation of monocytes (MO) and monocytic cells is modulated by 1α,25-dihydroxyvitamin D3 (Vitamin D3). Using mRNA Differential Display technology, Fritsche J et al. identified a gene induced in freshly isolated human blood MO cultured for 4 hours with Vitamin D3. This cDNA encodes a putative 16.5 kD protein with structural homology to yeast MMS2p and human CROC-1, defining a conserved Ubc-like protein family. This protein, called Ubiquitin E2 variant (UBE2V2) or MMS2, is required together with Ubc13 for RAD6/RAD18-dependent postreplicative DNA repair in yeast. These proteins play a central role in assembling K63-linked polyubiquitin chains that act as signals in DNA repair and IkBa kinase activation. Crystal structure analysis confirms that the Mms2/Ubc13 heterodimer is essential for DNA repair.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.