Thermo Fisher Scientific c-Myc Oncoprotein Recombinant Rabbit Monoclonal Antibody (MYC/7854R)
Recombinant rabbit monoclonal antibody recognizing c-Myc oncoprotein. Validated for WB, IHC (Paraffin), and IHC (PFA) applications. High specificity and reproducibility with protein A/G purification. Supplied as liquid, 1 mg/mL in PBS, for research use...
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | MYC/7854R |
| Immunogen | Recombinant fragment (around aa 1–200) of human MYC protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Wet ice |
Target Information
The c-Myc protein is a 62 kDa transcription factor encoded by the c-Myc gene on human chromosome 8q24. It is commonly activated in various tumor cells and plays a key role in cellular proliferation, differentiation, apoptosis, and cell cycle regulation. Phosphorylation at Thr58/Ser62 by kinases such as GSK3, CDK, ERK2, and JNK is associated with its regulatory function.
c-Myc is essential for vasculogenesis and angiogenesis in tumor development. Deregulated expression induces apoptosis and contributes to oncogenesis in multiple tissue types. Amplification of the c-Myc gene has been observed in lung, breast, and colon carcinomas, while translocation of the c-Myc locus is characteristic of Burkitt's lymphoma and other B-cell proliferative disorders.
Antibodies against c-Myc epitopes are widely used to detect Myc-tagged proteins and study oncogenic mechanisms related to cell growth and differentiation.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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