
Thermo Fisher Scientific DGKE Polyclonal Antibody
DGKE 단백질을 검출하기 위한 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. WB 및 ICC/IF에 사용 가능하며, 인간·마우스·랫트 반응성. 액상 형태로 1 mg/mL 농도, -20°C 보관. Affinity chromatography로 정제된 연구용 항체.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:1,000
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:100–1:500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human DGKE (Accession P52429), corresponding to amino acid residues N174–A224 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2850567 |
Product Specific Information
Antibody detects endogenous levels of total DGKE.
Target Information
Diacylglycerol (DAG) influences numerous cell signaling cascades by functioning as an intracellular activator of protein kinase C (PKC) and guanine nucleotide exchange factors. Intracellular DAG levels are regulated by diacylglycerol kinases (DGKs, DAGKs), which phosphorylate DAG to phosphatidic acid, maintaining cellular homeostasis.
Human DGKs are classified as follows:
- Type I: DGK-alpha (80 kDa), -beta (90 kDa), -gamma (90 kDa) — contain calcium-binding EF-hand motifs.
- Type II: DGK-delta (130 kDa), DGK-i (130 kDa) — contain N-terminal pleckstrin homology (PH) domains.
- Type III: DGK-epsilon (64 kDa) — contains no identifiable regulatory domains.
- Type IV: DGK-zeta (104 kDa), DGK-iota (130 kDa) — possess C-terminal ankyrin repeats.
- Type V: DGK-theta (110 kDa) — contains 3 cysteine-rich domains and a PH domain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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