Thermo Fisher Scientific Cre recombinase Monoclonal Antibody (GT10212)
Cre recombinase 단백질을 인식하는 마우스 단클론 항체로, Western blot과 Immunoprecipitation에 적합합니다. Virus에 반응하며, 단백질 G로 정제된 액상 형태입니다. 단기 4°C, 장기 -20°C 보관을 권장합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:20,000 | View 1 publication |
| Immunoprecipitation (IP) | 1:100–1:500 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Virus |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GT10212 |
| Immunogen | Recombinant protein conjugated synthetic peptide encompassing a sequence within the center region of Cre recombinase (exact sequence proprietary) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2735153 |
Product Specific Information
- Positive Control: Cre-tagged protein expressing HeLa, IPTG-induced cell lysate from E. coli expressing Cre recombinant proteins
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Cre recombinase is a 38 kDa DNA recombinase derived from the P1 bacteriophage. It is highly specific for a 34 bp DNA sequence (loxP) found in P1 DNA. It catalyzes site-specific recombination between two 34-base-pair loxP sites.
Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state. It forms a homotetramer when bound to DNA and belongs to the phage integrase family.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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