Thermo Fisher Scientific Cre recombinase Monoclonal Antibody (GT10212)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:20,000

View 1 publication 1 publication

Immunoprecipitation (IP)

1:100-1:500

-

Product Specifications

Species Reactivity

Virus

Published species

Not Applicable

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

GT10212

Immunogen

Recombinant protein conjugated synthetic peptide encompassing a sequence within the center region of Cre recombinase. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2735153

Product Specific Information

Positive Control: Cre-tagged protein expressing HeLa, IPTG-induced cell lysate from E. coli expressing Cre recombinant proteins

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Cre-recombinase is a 38 kDa DNA recombinase derived from the P1 bacteriophage. It is highly specific for a 34 bp DNA sequence (loxP) found in P1 DNA. It catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state. It is homotetramer when bound to DNA and belongs to the phage integrase family.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.