Thermo Fisher Scientific PYGL Polyclonal Antibody
Thermo Fisher Scientific의 PYGL Polyclonal Antibody는 인간, 생쥐, 랫트 반응성을 가지며 Western blot, IHC, ICC/IF, ELISA, IP에 적합합니다. Rabbit IgG 기반 비결합 항체로, 고순도 친화 크로마토그래피로 정제되었습니다. -20°C에서 보관하며 연구용으로만 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5 µg–4 µg antibody for 200 µg–400 µg extracts |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 690–847 of human PYGL (NP_0028543) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.425 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2855659 |
Product Specific Information
Immunogen sequence:
IGTMDGANVE MAEEAGEENL FIFGMRIDDV AALDKKGYEA KEYYEALPEL KLVIDQIDNG FFSPKQPDLF KDIINMLFYH DRFKVFADYE AYVKCQDKVS QLYMNPKAWN TMVLKNIAAS GKFSSDRTIK EYAQNIWNVE PSDLKISLSN ESNKVNGN
Target Information
This gene encodes a homodimeric protein that catalyzes the cleavage of alpha-1,4-glucosidic bonds to release glucose-1-phosphate from liver glycogen stores. The protein switches from inactive phosphorylase B to active phosphorylase A by phosphorylation at serine residue 15. Activity is regulated by allosteric effectors and hormonal controls. Humans have three glycogen phosphorylase genes expressed in liver, brain, and muscle. The liver isozyme supports systemic glycemic demands, while the brain and muscle isozymes serve local tissues. Mutations in the liver glycogen phosphorylase gene cause glycogen storage disease type VI (Hers disease), leading to moderate hypoglycemia, mild ketosis, growth retardation, and hepatomegaly. Alternative splicing produces multiple transcript variants.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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