Thermo Fisher Scientific Phospho-RelA (Ser468) Polyclonal Antibody
Phospho-RelA (Ser468) 폴리클로날 항체로 NF-κB p65 단백질의 인산화 상태를 검출. Western blot, IHC, IP 등 다양한 응용 가능. 항원 친화 크로마토그래피로 정제된 고순도 제품. 인체 및 마우스 시료에 반응하며 연구용으로 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Human v-rel reticuloendotheliosis viral oncogene homolog A surrounding serine 468 when serine 468 is phosphorylated |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Tris citrate/phosphate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
Target Information
Nuclear factor kB p65 (NF-κB p65) is encoded by the RELA gene and located on chromosome 11 in humans. NF-κB p65, also known as RelA, belongs to the Rel family of proteins and forms a heterodimer with p50 or p52 subunits. Upon TNF-α binding, IKK phosphorylates IκB, leading to its degradation and activation of NF-κB. NF-κB p65 plays a crucial role in transcription of the immunoglobulin κ gene in mature B-lymphoid cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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