Thermo Fisher Scientific MAPT Monoclonal Antibody (OTI6G3), TrueMAB

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:500

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

Tau is a neuronal microtubule-associated protein predominantly found on axons. It promotes tubulin polymerization and stabilizes microtubules.
The C-terminus binds axonal microtubules, while the N-terminus binds neural plasma membrane components, suggesting Tau functions as a linker between them.

Tau localization determines axonal polarity, with short isoforms contributing to cytoskeletal plasticity and long isoforms aiding stabilization.
Hyper-phosphorylated Tau forms paired helical filaments (PHF), a major component of neurofibrillary lesions in Alzheimer’s disease (AD).
This phosphorylation disrupts microtubule binding, leading to neuronal degeneration.
Kinases involved include GSK-3β, PKA, CDK5, and casein kinase II.
Hyper-phosphorylated Tau is also found in other CNS disorders such as Pick’s disease, frontotemporal dementia, corticobasal degeneration, and progressive supranuclear palsy.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.