Thermo Fisher Scientific IL-10 Monoclonal Antibody (JES5-2A5), Functional Grade, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

-

View 1 publication 1 publication

ELISA (ELISA)

-

View 6 publications 6 publications

Neutralization (Neu)

Assay-Dependent

View 8 publications 8 publications

Functional Assay (Functional)

Assay-Dependent

View 5 publications 5 publications

Inhibition Assays (IA)

-

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Published species

Mouse

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), Functional Grade, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

JES5-2A5

Conjugate

Functional Grade Functional Grade Functional Grade

View additional formats

• Biotin
• Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_2866230

Product Specific Information

Description: The JES5-2A5 antibody reacts with mouse interleukin-10 (IL-10).The JES5-2A5 antibody is a neutralizing antibody.Mouse IL-10 is an 18 kDa factor also known as Cytokine Synthesis Inhibitory Factor (CSIF).In the mouse, Th2 cells, B1 cells, macrophages, and keratinocytes are the major cell subsets that produce IL-10.IL-10 inhibits synthesis of Th1 cytokines and proliferation of T cells, and acts as a costimulatory signal for mast cells, developing thymocytes and the Th2 response.

Applications Reported:The JES5-2A5 antibody has been reported for use in ELISA, ELISPOT, neutralization of IL-10 bioactivity, and intracellular staining for flow cytometric analysis.

Applications Tested: The Functional Grade Purified JES5-2A5 antibody has been tested by LAL assay to verify low endotoxin levels, by ELISA, and in bioassay for neutralization of mouse IL-10 bioactivity.

The JES5-2A5 antibody at 0.004 µg/mL has been found to inhibit by 50% the biological effects of 1.0 ng/mL mouse IL-10 in an MC/9 cell proliferation assay. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the BestProtocols® section.

The biotinylated JES5-2A5 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse interleukin-10 (IL-10) in combination with the purified JES5-16E3 (14-7101) antibody for capture and recombinant mouse IL-10 (14-8101) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 4000 pg/mL - 30 pg/mL should be included in each ELISA plate.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level:Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation:Less than 10%, as determined by HPLC.

Target Information

IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.