Takara mRNA Cap 2`-O-Methyltransferase

mRNA Cap 2`-O-Methyltransferase uses S-adenosylmethionine (SAM) as a methyl donor to add a methyl group at the 2’-O position of the first nucleotide at the 5’ end of Cap-0 mRNA, resulting in Cap-1 structure. Cap-1 RNA structure may improve evasion of innate immune response and promote translation in vivo.

Use Vaccinia Capping Enzyme (Cat. # 2460) together with this enzyme to produce Cap-1 RNA directly from uncapped RNA (5’-triphosphate RNA) in a single post-transcriptional reaction.