Thermo Fisher Scientific SIRT1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:5,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 448-747 of human SIRT1 (NP_0363702) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SIRT1,uniProtId:Q96EB6-1,ncbiNodeId:9606,antigenRange:448-747,antigenLength:747,antigenImageFileName:PA5-116530_SIRT1_Q96EB6-1_Rabbit.svg,antigenImageFileNamePDP:PA5-116530_SIRT1_Q96EB6-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2.92 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2901161

Product Specific Information

Positive Samples: 293T, HeLa, Jurkat

Immunogen sequence: VALIPSSIPH EVPQILINRE PLPHLHFDVE LLGDCDVIIN ELCHRLGGEY AKLCCNPVKL SEITEKPPRT QKELAYLSEL PPTPLHVSED SSSPERTSPP DSSVIVTLLD QAAKSNDDLD VSESKGCMEE KPQEVQTSRN VESIAEQMEN PDLKNVGSST GEKNERTSVA GTVRKCWPNR VAKEQISRRL DGNQYLFLPP NRYIFHGAEV YSDSEDDVLS SSSCGSNSDS GTCQSPSLEE PMEDESEIEE FYNGLEDEPD VPERAGGAGF GTDGDDQEAI NEAISVKQEV TDMNYPSNKS

Target Information

NAD-dependent protein deacetylase sirtuin-1 (SIRT1) links transcriptional regulation directly to intracellular energetics and participates in the coordination of several separated cellular functions such as cell cycle, repsonse to DNA damage, metabolism, apoptosis, and autophagy. SIRT1 can modulate chromatin function through deacetylation of histones and can promote alterations in the methylation of histone and DNA, leading to transcriptional repression. It is essential in skeletal muscle cell differentiation and in response to low nutrients mediates the inhibitory effect on skeletal myoblast differentiation which also involves 5`-AMP-activated protein kinase (AMPK) and nicotinamide phosphoribosyltransferase (NAMPT).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.