Thermo Fisher Scientific SIRT1 Polyclonal Antibody
SIRT1 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal 항체입니다. Western blot, IHC, ELISA 등 다양한 응용에 적합하며, 고순도 Affinity chromatography로 정제되었습니다. Human, Mouse, Rat 시료 반응성이 있으며, 액상 형태로 -20°C 보관이 권장됩니다.
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Thermo Fisher Scientific SIRT1 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 448–747 of human SIRT1 (NP_0363702) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2.92 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2901161 |
Product Specific Information
- Positive Samples: 293T, HeLa, Jurkat
- Immunogen Sequence:
VALIPSSIPH EVPQILINRE PLPHLHFDVE LLGDCDVIIN ELCHRLGGEY AKLCCNPVKL SEITEKPPRT QKELAYLSEL PPTPLHVSED SSSPERTSPP DSSVIVTLLD QAAKSNDDLD VSESKGCMEE KPQEVQTSRN VESIAEQMEN PDLKNVGSST GEKNERTSVA GTVRKCWPNR VAKEQISRRL DGNQYLFLPP NRYIFHGAEV YSDSEDDVLS SSSCGSNSDS GTCQSPSLEE PMEDESEIEE FYNGLEDEPD VPERAGGAGF GTDGDDQEAI NEAISVKQEV TDMNYPSNKS
Target Information
NAD-dependent protein deacetylase sirtuin-1 (SIRT1) links transcriptional regulation directly to intracellular energetics and participates in coordinating cellular functions such as cell cycle, DNA damage response, metabolism, apoptosis, and autophagy. SIRT1 modulates chromatin function via histone deacetylation and influences histone and DNA methylation, leading to transcriptional repression. It plays a key role in skeletal muscle cell differentiation and nutrient response, involving AMPK and NAMPT pathways.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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