Thermo Fisher Scientific HP1 alpha Polyclonal Antibody
HP1 alpha 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal 항체로, WB, ICC/IF, IP, ChIP 등 다양한 응용에 적합합니다. 고농도(3.07 mg/mL) 액상 형태로 제공되며, HeLa 등 여러 세포주에서 검증되었습니다. 연구용 전용 제품입니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Immunoprecipitation (IP) | 1:50–1:200 |
| ChIP assay (ChIP) | 1:20–1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1–191 of human CBX5 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 3.07 mg/mL |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2901156 |
Product Specific Information
Positive Samples: HeLa, K-562, SW620, A-673, SH-SY5Y, 293T
Target Information
Heterochromatin protein-1 (HP1) is a methyl-lysine binding protein localized at heterochromatin sites, where it mediates gene silencing. Mammalian methyltransferases that selectively methylate histone H3 on lysine-9 generate a binding site for HP1 proteins, a family of heterochromatic adaptor molecules implicated in both gene silencing and supranucleosomal chromatin structure. High-affinity in vitro recognition of a methylated histone H3 peptide by HP1 requires a functional chromodomain. Thus, the HP1 chromodomain is a specific interaction motif for the methyl epitope on lysine-9 of histone H3. In vivo, heterochromatin association of HP1 proteins is lost in Suv39h double-primary mouse fibroblasts but is restored after reintroduction of a catalytically active SUV39H1 HMTase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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